On-Line measurement of molecular weight and radius of gyration of polystyrene in a good solvent and in a theta solvent measured with a two-angle light scattering detector

On-line two-angle (15 and 90) light scattering measurements with a gel permeation chromatograph for linear and branched polystyrene in tetrahydrofuran (a good solvent) and in trans-decalin (a theta solvent) were made and compared with data from a multi-angle light scattering detector and literature values. Theoretically, weight-average molecular weight and the radius of gyration Rg can be determined accurately in the range where Rgk is less than 1.2 (rod) ~ 1.7 (random coil); here, k is the absolute value of the scattering vector for a right angle detector with the Berry square root method. Molecular weight dependence of the radius of gyration obtained from the two-angle light scattering detector for linear and branched polystyrenes under different thermodynamic conditions were measured and found to be almost the same as values measured with a multi-angle light scattering detector and literature values in the appropriate range of molecular weight.